G GRANT CRFT System: Precision Cryopreservation for iPSCs & Organoids
Preserve Your iPSCs & Organoids with Precision. CRFT provides programmable, controlled-rate freezing and thawing without liquid nitrogen ensuring optimal viability for delicate samples.
Applications
iPSCs
- Optimal cooling between –0.3 to –1.8 °C/min enhances survival
- Three-zone strategies (fast–slow–fast) fine-tune dehydration and nucleation balance
Organoids
- Slow, controlled freezing better preserves organoid architecture versus vitrification alone
- Precise thawing reduces thermal stress and improves post-thaw recovery
About
Why CRFT for iPSC & Organoid Cryopreservation?
Recent studies highlight that well-controlled freeze/thaw protocols - especially those leveraging programmable controlled-rate systems - achieve >94% viability post-thaw, compared to ~60% with conventional methods
Delicate stem cell and organoid structures need gradual, consistent temperature control during cryopreservation. CRFT offers programmable freeze/thaw profiles that safeguard structural and functional integrity minimising ice damage and maximizing recovery.
Technical highlights:
- Temperature Range: From -100 °C to +30 °C (Temperature range varies depending on the installed head)
- Cooling/Thawing Rate: Up to ±10 °C/min with ±0.5 °C accuracy
- Plate and Sample Adaptability: Handles SBS plates, cryovials, straws via modular heads
- Data Logging: Continuous monitoring and exportable audit trail
Key Benefits:
- Liquid-nitrogen-free operation with low running costs and safer handling
- Real-time data logging for full traceability and reproducibility
- Interchangeable heads accommodate cryovials, plates, straws, and more
- Automated-friendly design for seamless integration into workflows
- Precise control: cooling and thawing at up to ±10 °C/min, ±0.5 °C accuracy, ±1.0 °C uniformity
Specifications
| Dimensions (mm) – wdh |
345 x 290 x 380 (lid off) / 460 (lid on) |
| Weight (kg) |
5kg |
| Max plate temp (°C) |
30°C |
| Min plate temp (°C) |
-100°C* |
| Temp accuracy |
+/- 0.5°C |
| Temp stability |
+/- 0.1°C |
| Temp uniformity |
+/- 1.0°C Ref |
| Max cooling rate |
10°C min/min |
| Max thawing rate |
10°C/min from -100°C |
| Max temp stabilisation time |
5 mins |
| Plug type |
UK, EU or US |
| Power supply |
100-240V, 50-60Hz (24V DC unit supply) |
| Warranty |
2 years |
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